The decline of grain elevators in Canada has increased reliance on local truck-based grain transportation, raising concerns over economic costs, environmental impacts, and rural sustainability. This study explores the potential of shortline rail as a sustainable alternative for grain transportation from farms to elevators, adopting the triple-bottom-line sustainability framework"”economic, environmental, and social sustainability. The research aims to develop a comprehensive sustainability assessment framework tailored to shortline rail networks, integrating quantitative sustainability indicators and qualitative stakeholder feedback. The proposed framework includes indicator forecasting, stakeholder analysis, engagement strategies, and case studies to evaluate the current grain transportation system and the viability of shortline rail networks in improving sustainability. By assessing cost efficiency, carbon footprint reduction, logistical feasibility, and socio-economic benefits, this study highlights shortline rail's potential to enhance rural economic development, reduce transportation bottlenecks, and strengthen community resilience. Findings will contribute to evidence-based policy recommendations for optimizing Canada's grain supply chains while fostering long-term sustainability in the agricultural transportation sector.